The objectives of this study were to 1 determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2 evaluate the potential of timeresolved laserinduced fluorescence spectroscopy trlifs as a diagnostic instrument for head and neck squamous cell carcinoma hnscc. Tcspc requires a defined start, provided by the electronics steering the laser pulse or a photodiode, and a defined stop. Home arteriosclerosis, thrombosis, and vascular biology vol. In the case of oral ingestion of radioactive contaminants, the first contact medium is saliva in the mouth. Laserinduced fluorescence spectroscopy lifs is a useful tool for. Accumulation of numerous macrophages in the fibrous cap is a key identifying feature of plaque inflammation and vulnerability. Scime june, 2010 international conference on spectral line shapes. Laserinduced fluorescence has developed enormously since its inception. Timeresolved, laserinducedfluorescence measurements of ion flows and temperatures in a helicon plasma source earl e.
All references cited in this specification are incorporated by reference as if all in their. Keywords raman spectroscopy, laserinduced breakdown spectroscopy, libs, laserinduced fluorescence spectroscopy, lif, timeresolved spectroscopy, remote spectroscopy, standoff spectroscopy, hyphenated techniques, planetary science field instrument concepts. Timeresolved laserinduced fluorescence spectroscopy trlfs was used to study the speciation of uraniumvi at very alkaline ph 11, at room temperature and in the absence of co2. Ultrafast laser technology has moved from dye lasers in the 1980s to solid state lasers, primarily ti. Pdf highly time resolved measurements of oh during. These include a fast digitizing oscilloscope, a small nanosecond pulsed laser, and a fast photomultiplier tube, all assembled on a table no black. Discrimination of human coronary artery atherosclerotic lipidrich lesions by timeresolved laserinduced fluorescence spectroscopy.
Strong fluorescence activity of these complexes predetermine trlfs method. Characterization of atherosclerotic lesions with laser. Timeresolved laser induced fluorescence spectroscopy. Several techniques have been developed recently for performing timeresolved laserinduced fluorescence lif measurements in oscillating plasmas. Ambient air spectra were recorded by tuning the laser wavelength over a spectral region covering the q1, q2, and p111 rotational transitions of the 00 band in the ax system of.
Resonance fluorescence excitation of diatomic aluminum monoxide alo and triatomic dialuminum monoxide al2o was used to identify these chemical intermediates. Pdf our developments of the timeresolved laserinduced fluorescence trlif. With applications in biophysics and physical chemistry. Laserinduced fluorescence spectroscopy lif sciencedirect. Lasers in farred to nir are usually chosen as light sources for raman spectroscopy. Time resolved laser induced fluorescence spectroscopy at. Timeresolved laserinduced fluorescence of arterial wall. This may involve laser induced fluorescence to detect the tumor or diagnosis of meningioma by time resolved fluorescence spectroscopy. Pdf development of timeresolved laserinduced fluorescence. Technical progress in laser technology continues unabated. Usually ultrafast lasers with femtosecond and picosecond durations are used for laser tissues welding in nir, timeresolved fluorescence spectroscopy and optical timegating imaging. Timeresolved laser induced fluorescence spectroscopy trlifs can be used to differentiate various tissue types in vivo, based on the. The performance of optical coatings for high power duvvuv laser applications depends amongst others on residual.
Plaque with dense inflammatory cells, including macrophages, thin fibrous cap and superficial necroticlipid core is thought to be pronetorupture. Statistical analysis of the data indicates the clinical potential of this approach for detecting esophageal dysplasia. The objectives of this study were to evaluate the ability of timeresolved, laserinduced fluorescence spectroscopy trlifs to serve as a noninvasive diagnostic technique for squamous cell carcinoma in the hamster cheek pouch model and to determine whether timeresolved, fluorescencederived parameters can add diagnostic value to fluorescence. Laser induced fluorescence spectroscopy lifs is a useful tool for characterization of biological tissues and offers potential for in vivo diagnosis of diseased tissues and the optimization of therapeutic. Measurement and analysis of timeresolved emission spectra following laserinduced optical breakdown liob allows us to characterize microplasmas that contain carbon. Objective to investigate the benefit of using timeresolved, laserinduced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity. Laser induced fluorescence spectroscopy an overview. Development of a novel time resolved laser induced fluorescence technique christopher j.
Laserinduced fluorescence in spectroscopy, dynamics, and. Our results confirm that timeresolved raman and fluorescence spectroscopy is a very promising method for the exploration of planetary surface, in particular for the characterization of alteration andor biorelated samples phosphates, carbonates, sulfates, clays and the detection of organics. Timeresolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. The present invention provides a system for characterizing a biological sample by analyzing the fluorescence emission from the biological sample upon excitation and a method for using the same. Timeresolved laserinduced fluorescence spectroscopy trlifs is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix ecm produced by putative stem cells pla cells derived. Temporal resolution in pumpprobe spectroscopy is limited by the laser pulse durations used. The system includes a laser light source, a collection fiber, a demultiplexer, and an optical delay device.
In this case, at ph 11, two different fluorescence lifetimes appeared, which were attributed to the species uo2oh3. The instrumentation in timeresolved spectroscopy evolves at a very rapid pace. We report a timeresolved laserinduced fluorescence spectroscopy trlifs technique for detection of such markers of plaque vulnerability in human plaques. To gain a first insight into the interaction of radioactive contaminants in human saliva, the speciation of curium cmiii and europium euiii, i. The objectives of this study were to 1 determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2 evaluate the potential of timeresolved laserinduced fluorescence. Yag laser pulses used to excite various biomolecules. Detection of ruptureprone atherosclerotic plaques by time. Characterization of atherosclerotic lesions with laserinduced timeresolved fluorescence spectroscopy, proc. Picosecond lasers can be used as excitation light sources for timeresolved fluorescence. Jp2016512891a timeresolved laserinduced fluorescence. Discrimination of human coronary artery atherosclerotic. Laser induced fluorescence has developed enormously since its inception. Timeresolved laserinduced fluorescence spectroscopy as a. One of the primary applications is characterizing plasma fluctuations in devices like hall thrusters used for space propulsion.
In the technique of laserinduced fluorescence, or lif, a laser is. In the following, we will concentrate on the equipment issues of ultrafast spectroscopy. Laserinduced fluorescence lif fluorescent dyes molecules can absorb light at one frequency and subsequently reemit fluoresce light at a different frequency. Timeresolved fluorescence studies on protoporphyrin ix. Fluorescence spectroscopy is typically divided into steadystate spectrallyresolved or intensity measurements and timeresolved timedomain and frequencydomain techniques. Among these techniques, timeresolved taserinduced fluorescence spectroscopytrlfs is especially promising, since an intense laser light source allows. Picosecond lasers can be used as excitation light sources for timeresolved fluorescence spectroscopy and raman spectroscopy studies.
Time resolved laser induced fluorescence spectroscopy trlifs can be used to differentiate various tissue types in vivo, based on the intrinsic fluorescent time decay properties of these tissues. Uranylsulfate complexes namely uo2so4, uo2so422 a uo2so434 are a subejct to study for their appearance in radioactivewaste leach by subterrestrial water. Time resolved laser induced fluorescence of normal and atherosclerotic coronary. Pdf timeresolved laser fluorescence spectroscopy trlfs is a useful. Search the leading research in optics and photonics applied research from spie journals, conference proceedings and presentations, and ebooks. Here, the fluorescence of a sample is monitored as a function of time after excitation by a flash of light. Gallimore2 1applied physics, university of michigan 2aerospace engineering, university of michigan introduction we have developed a technique using laser induced fluorescence lif to measure the time resolved. Furthermore, timeresolved fluorescence spectroscopy represents a potential tool to aid in. Scientists are using laserinduced fluorescence to assess. Compared to pumpprobe spectroscopy techniques such as transient absorption, the data interpretation of fluorescence up conversion is straightforward as only emission is measured. A preliminary study of contemporary binders by time. Time resolved fluorescence spectroscopy an overview.
This system is based on the tunable 320 nm to 475 nm nd. Introduction most nonintrusive optical diagnostics of flames are 1d or 2d measurements. This is a pdf file of an unedited manuscript that has been. Timeresolved laserinduced fluorescence diagnostics for. Design the carcinogen 7,12dimethylbenzaanthracene dmba was applied to 1 cheek pouch of 19 hamsters. Uranium speciation studies at alkaline ph and in the. A new timeresolved laserinduced fluorescence spectrometry trlfs data acquisition procedure applied to the uranylphosphate system article pdf available in radiochimica acta 953.
Us10288567b2 timeresolved laserinduced fluorescence. Introduction a major problem encountered in the conservation of contemporary paintings is the identification of their material composition. Life science timeresolved fluorescence measurements of the fluorescence lifetime. Timeresolved, laserinduced fluorescence measurements of. Nondestructive measurements of liquid and solid samples in real time selectivity. Fast 2d laserinduced fluorescence spectroscopy mapping. Laser induced fluorescence spectroscopy, diesel fuel, biodiesel, fuel sensor.
The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Employing timeresolved luminescence experiments could help. Timeresolved laserinduced fluorescence spectroscopy. Pdf spectroscopic properties of uraniumvi minerals. Timeresolved resonance fluorescence spectroscopy for. Pdf applications of timeresolved laser fluorescence. Timeresolved 3d oh planar laserinduced fluorescence. Presence of hydrogen in the sample will result in balmer series emissions, and particularly the emission will be.
Developed first as a spectroscopic method for its own sake, it soon found application in time resolved investigations reaching into the ultrafast regime. Several spectroscopic methods specially the timeresolved laserinduced fluorescence spectroscopy and the xrayabsorption spectroscopy were used for the determination of the uranium speciation in. In experiments, the dyes are excited by laser light whose frequency closely matches the excitation frequency of the dye. Timeand wavelengthresolved fluorescence spectroscopy for. It has been applied in the identification of fossil fuels hegazi et al. Tropospheric hydroxyl radical oh concentrations were measured by laserinduced fluorescence lif during the popcorn field campaign in august 1994 at a rural site in the north east of germany. Our developments of the timeresolved laserinduced fluorescence trlif detection system for biomolecules are presented. Timeresolved spectroscopy an overview sciencedirect. Fluorescence up conversion offers fsresolved dynamics information over a. Trlifs data showed the presence of several distinct emission components. A method of timeresolved laserinduced fluorescence spectroscopy is suitable for remote sensing applications using an intense pulsed qswitched laser. Timeresolved spectroscopy diagnostic of laserinduced.
Laser induced fluorescence spectroscopic sensor for. Examples are particle imaging velocimetry piv, raman spectroscopy, coherent antistokes raman spectroscopy, cars, and planar laser induced fluorescence plif. Steadystate and timeresolved fluorescence properties of the hrpdesfe adduct wele examined. Timeresolved fluorescence spectroscopy as a diagnostic. Most studies using fluorescence spectroscopy in head and neck tumors focus on the spectral intensity measurements. In this work, timeresolved resonance fluorescence spectroscopy was studied as a promising tool for the study of chemical reactions in laserinduced plasmas. This study investigates the use of timeresolved laserinduced fluorescence spectroscopy trlifs as a potential tool for detection of macrophage foam cells in the intima of atherosclerotic plaques. Spectroscopy in the sky laser beams shoot into the colorado night sky, probing the secrets of the mesopause region of the atmosphere. Highly time resolved measurements of oh during popcorn using laserinduced fluorescence spectroscopy article pdf available in journal of atmospheric chemistry 311. The long exposure revealed the location of the north star as well as low laser power about 0. Timeresolved and steadystate autofluorescence were measured in 41 patients with barretts esophagus using excitation wavelengths of 337 and 400 nm. The detection part is the streak system for fluorescence lifetime spectroscopy hamamatsu, japan. Timeresolved laserinduced fluorescence spectroscopy as a diagnostic. Time resolved laser induced fluorescence spectroscopy is a modern laser spectroscopic method used for studying chemical forms species in which elements are present even in trace concentrations.
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